assessment of the relative level of tnf-α gene expression in raw264.7 cells

background and objective: recent studies have shown that not only the quantity, but also the quality of dietary fatty acids can affect lipid parameters. elaidic acid is a major hydrogenated by-product of vegetable oil play a role in the development of cardiovascular disease, especially atherosclerosis, through changing lipid profile and increase in inflammation and inflammatory factors. on the other hand, the role of tnf-α is evident in inflammatory pathways and inflammation outcomes, which are involved in the initiation of atherosclerosis. therefore, in the current study the effect of elaidic acid on tnf-α gene expression, was investigated.   method: raw264.7 cells were treated with 100 and 200 μm of elaidic acid for 12 h. then, expression of tnf-α gene was assessed after rna extraction and cdna synthesis.  results:  tnf-α gene expression in raw264.7 macrophage cell line showed no significant difference for both concentrations compared to the control.  conclusion: these results indicated that elaidic acid affect the development and acceleration of atherosclerosis in other ways than through tnf-α nuclear receptor.